Бібліотека Вінницького національного медичного університету ім. М.І.Пирогова

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ATP-sensitive potassium transport in rat brain mitochondria is highly sensitive to mK ATP channels openers: a light scattering study / O. V. Akopova [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P5-14

MeSH-головна:
МОЗГ ГОЛОВНОЙ -- BRAIN (воздействие облучения, метаболизм, патофизиология)
МИТОХОНДРИИ -- MITOCHONDRIA (воздействие облучения, метаболизм)
КАЛИЙ -- POTASSIUM (метаболизм)
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: The aspects of ATP-sensitive K+ transport regulation by mitochondrial K+,ATP-sensitive (mKATP) channels openers are important for understanding the properties of these channels. The effect of KATP channels openers (KCOs) diazoxide and pinacidil on ATP-sensitive K+ transport in isolated brain mitochondria was studied in the absence and the presence of MgATP using light scattering technique. Without MgATP we observed high sensitivity of ATP-sensitive K+ transport to both drugs with full activation at ≤ 0.5 µM. ATP-sensitive K+ transport was specifically blocked by ATP in the presence of Mg2+. Neither Mg2+ nor ATP affected Vmax of ATP-sensitive K+ transport activated by KCOs, but MgATP shifted the activation curve to micromolar scale. The blockage of ATP-sensitive K+ transport by KATP channels blockers glibenclamide and 5-hydroxydecanoate in the absence and the presence of MgATP proved the sensitivity of ATP-sensitive K+ transport to the blockers of mKATP channel. Full activation of mKATP channel by diazoxide and pinacidil on sub-micromolar scale in the absence of MgATP was shown. The sensitivity of ATP-sensitive K+ transport to the known modulators of mKATP channel (diazoxide, pinacidil, glibenclamide, 5-HD and MgATP) proved the identity of ATP-sensitive K+ transport with mKATP channel activity. Based on our studies, we hypothesized that mKATP channel might comprise high affinity sites for KCOs binding screened by MgATP. The results of this work reveal novel not described earlier aspects of the regulation of ATP-sensitive K+ transport by mKATP channels openers, important for understanding of mKATP channel properties
Дод.точки доступу:
Akopova, O. V.
Kolchinskaya, L. I.
Nosar, V. I.
Smirnov, A. N.
Bratus, L. V.

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Kinetics of interaction between polyreactive immunoglobulins and antigen / S. A. Bobrovnik [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P15-22

MeSH-головна:
ИММУНОГЛОБУЛИНЫ -- IMMUNOGLOBULINS (метаболизм)
АНТИГЕНЫ -- ANTIGENS (метаболизм)
Анотація: A number of experimental kinetics curves of mice polyreactive immunoglobulins (PRIGs) binding to ovalbumin immobilized on immunologic plates were obtained at different temperatures. Analysis of these curves allowed us to conclude that the model of PRIGs interaction with antigens proposed by us earlier and consisted on PRIGs activation (i.e. exposition of hydrophobic patches on PRIGs surface) and either sequential binding to antigen or inactivation was is in a good agreement with the experimental data obtained in this study. We have designed a method of the rate constants evaluation from experimental binding curves. It was found that the rate constant of the activated PRIGs binding to immobilized antigen did not depend on temperature. The rate constant of PRIGs activation occurred to be depend on temperature more strongly than the rate constant of PRIGs inactivation. We have concluded from the acquired dependences that at 37°С the number of activated PRIGs was 15 times higher than that at 0°С
Дод.точки доступу:
Bobrovnik, S. A.
Ogloblya, O. V.
Demchenko, M. O.
Komisarenko, S. V.

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Selected 5-amino-1-aryl-1H-1,2,3-triazole scaffolds as promising antiproliferative agents / N. Pokhodylo [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P23-32

MeSH-головна:
ПРОТИВООПУХОЛЕВЫЕ СРЕДСТВА -- ANTINEOPLASTIC AGENTS (химический синтез, химия)
ТРИАЗОЛЫ -- TRIAZOLES (химия)
ХИНАЗОЛИНОНЫ -- QUINAZOLINONES (химия)
ТИАЗОЛЫ -- THIAZOLES (терапевтическое применение)
Анотація: Development of a new effective drugs with low side effects and definite chemical characteristics needs indentification of bioactive scaffolds for further structural optimization. New synthesized derivatives of 4-hetaryl-5-amino-1-aryl-1H-1,2,3-triazoles and 3H-[1,2,3]triazolo[4,5-b]pyridines were tested for anticancer activity using 60 human tumor cell lines within 9 cancer types. The selective influence of (5-amino-1H-1,2,3-triazol-4-yl)quinazolin-4(3H)-ones: 2-(5-amino-1-(4-chlorophenyl)-1H-1,2,3-triazol-4-yl)quinazolin-4(3H)-one and 2-(5-amino-1-phenyl-1H-1,2,3-triazol-4-yl)-6-bromoquinazolin-4(3H)-one on ovarian cancer OVCAR-4 cells with growth percentage (GP) = -4.08 and 6.63%, respectively, was found. The derivative 5,7-diamino-3-(3-(trifluoromethyl)phenyl)-3H-[1,2,3]triazolo[4,5-b]pyridine-6-carbonitrile possessed high activity towards lung cancer EKVX cells (GP = 29.14%). The compounds were shown to be less toxic than doxorubicin towards non-tumor human embryonic kidney cells of HEK293 line. Thus, the results of our study confirm the anticancer potential of compounds based on 5-amino-1-aryl-1H-1,2,3-triazoles scaffolds and their fused polycyclic derivatives
Дод.точки доступу:
Pokhodylo, N.
Shyyka, O.
Finiuk, N.
Stoika, R.

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Shahouzehi, B.
L-carnitine administration effects on AMPK, APPL1 and PPARγ genes expression in the liver and serum adiponectin levels and HOMA-IR in type 2 diabetes rat model induced by STZ and nicotinamide / B. Shahouzehi, H. Fallah, Y. Masoumi-Ardakani // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P33-40

MeSH-головна:
ПЕЧЕНЬ -- LIVER (действие лекарственных препаратов, патофизиология, повреждения)
КАРНИТИН -- CARNITINE (генетика, фармакокинетика)
ГЕННАЯ ЭКСПРЕССИЯ -- GENE EXPRESSION (действие лекарственных препаратов)
АДИПОНЕКТИН -- ADIPONECTIN (фармакокинетика)
КРОВИ СЫВОРОТКА -- SERUM (действие лекарственных препаратов, метаболизм)
СТРЕПТОЗОТОЦИН -- STREPTOZOCIN (вредные воздействия)
НИКОТИНАМИД -- NIACINAMIDE (вредные воздействия)
ДИАБЕТ САХАРНЫЙ -- DIABETES MELLITUS (генетика, метаболизм, патофизиология)
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: Diabetes is a chronic disease and a public health problem globally. L-Carnitine is synthesized in the liver, promotes fatty acids oxidation and currently is used as a supplement against weight gain. Carnitine level is found to be reduced in diabetic patients and to be beneficial as a supplement at diabetes, but the mechanisms of this effect is not fully understood. Therefore, we evaluated the oral L-carnitine supplementation on expression of AMP-activated protein kinase (AMPK), peroxisome proliferator-activated receptor gamma (PPARγ), adaptor protein APPL1 genes in the liver and insulin and adiponectin levels in the serum of diabetic rats. Rats were randomly divided into three groups (n = 8) as follow: group 1 – control without any treatment, group 2 – diabetic control rats which received STZ (45 mg/kg) and nicotinamide (200 mg/kg) by i.p. injection, group 3 – diabetic rats which received 600 mg/kg/day carnitine orally for 35 days. It was found that L-carnitine supplementation reduced the level of fasting glucose compared to that in control and diabetic groups (P = 0.001, P = 0.0001 respectively) and increased adiponectin level compared to diabetic nontreated rats (P = 0.0001). Homeostasis model assessment of insulin resistance (HOMA-IR) was significantly increased in the diabetic group and reduced in the group that received L-carnitine. These promising beneficial effect of L-carnitine on the type 2 diabetes in rats’ model was shown to be conducted through the up-regulation of AMPK, PPARγ and APPL1 genes expression in the liver and elevation of serum adiponectin level
Дод.точки доступу:
Fallah, H.
Masoumi-Ardakani, Y.

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Gulevskyy, A. K.
Cryoprotective agents affect amino acids incorporation into total proteins in cells of lymphoid organs and liver of experimental animals / A. K. Gulevskyy, Yu. S. Akhatova, A. Yu. Nikolchenko // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P41-49

MeSH-головна:
ПЕЧЕНЬ -- LIVER (действие лекарственных препаратов)
ЛИМФОИДНАЯ ТКАНЬ -- LYMPHOID TISSUE (действие лекарственных препаратов)
КРИОПРОТЕКТОРЫ -- CRYOPROTECTIVE AGENTS (фармакокинетика)
АМИНОКИСЛОТЫ -- AMINO ACIDS (фармакокинетика)
ЭУКАРИОТЫ -- EUKARYOTA
Анотація: The effect of penetrating (glycerol, DMSO) and poorly penetrating (PEG-400) cryoprotective agents on labeled amino acids incorporation into de novo synthesized proteins in the cells of mice thymus, lymph nodes, spleen and cell-free rat liver extract was studied. Cryoprotective agents within the range of concentrations which provide a cryoprotective effect were found to inhibit significantly protein synthesis in cell-free systems under investigation. The most effective inhibition was exerted by polymeric cryoprotective agent PEG-400. Cryoprotective agents more efficiently inhibited protein synthesis at a cell level as compared with that in cell-free system that was likely associated with their effect on amino acids transport system. An inhibitory effect of cryoprotective agents on the protein synthesizing apparatus of cells was determined to be Mg2+-dependent and reversible
Дод.точки доступу:
Akhatova, Yu. S.
Nikolchenko, A. Yu.

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ERN1 dependent regulation of TMED10, MYL9, SPOCK1, CUL4A and CUL4B genes expression at glucose and glutamine deprivations in U87 glioma cells / O. H. Minchenko [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P50-61

MeSH-головна:
ГЛИОМА -- GLIOMA (генетика, метаболизм)
ГЛУТАМИН -- GLUTAMINE (дефицит)
ГЛЮКОЗА -- GLUCOSE (дефицит)
Анотація: It was shown previously that inhibition of ERN1 (endoplasmic reticulum to nucleus signaling 1) pathway, a central mediator of the unfolded protein response, leads to suppression of tumor growth through down-regulation of key pro-proliferative and up-regulation of tumor suppressor factors and modifies the sensitivity of these genes to glucose and glutamine deprivation. However, the executive mechanisms of ERN1 mediated control of glioma cell proliferation are not yet known. The goal of this study was to estimate the effect of glucose and glutamine deprivations on expression of cancer related genes in glioma U87 cells at ERN1 signaling inhibition for evaluation of their possible significance in ERN1 mediated control of glioma cell proliferation. We have studied the effect of glucose and glutamine deprivations on the expression level of cancer related genes encoding TMED10 (transmembrane p24 trafficking protein 10), MYL9 (myosin, light chain 9, regulatory), SPOCK1 (sparc/osteonectin, cwcv and kazal-like domains proteoglycan 1), CUL4A (cullin 4A), and CUL4B in U87 glioma control cells and cells with ERN1 knockdown. It was shown that at glucose deprivation the expression level of MYL9, SPOCK1 and CUL4B genes was significantly up-regulated in control glioma cells. ERN1 knockdown modified the sensitivity to glucose deprivation of all studied genes except TMED10 gene. At glutamine deprivation the expression of MYL9, CUL4A and CUL4B genes was shown to be up-regulated in control glioma cells. The sensitivity of MYL9, TMED10 and CUL4B gene expression to glutamine deprivation in glioma cells with ERN1 knockdown was significantly modified, while CUL4A and SPOCK1 gene expression did not respond to ERN1 inhibition. The present study demonstrates that glucose and glutamine deprivation affected the expression of the most studied genes in a specific manner and that inhibition of ERN1 signaling preferentially modified their expression at glucose and glutamine deprivation
Дод.точки доступу:
Minchenko, O. H.
Hnatiuk, O. S.
Tsymbal, D. O.
Viletska, Y. M.
Danilovskyi, S. V.
Halkin, O. V.
Kryvdiuk, I. V.
Rudnytska, O. V.

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DNA loop domain rearrangements in blast transformed human lymphocytes and lymphoid leukaemic Jurkat T cells / K. Afanasieva [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P62-69

MeSH-головна:
ЛИМФОЦИТЫ -- LYMPHOCYTES (метаболизм)
ЛЕЙКОЗ T-КЛЕТОЧНЫЙ -- LEUKEMIA, T-CELL (генетика)
Анотація: Chromatin loops are important elements of both chromatin higher-order structure and transcription regulation system. Our previous works have shown that several features of the loop domain organization could be investigated by single cell gel electrophoresis (the comet assay) using the kinetic approach. In this study we applied this technique to study DNA loop domain organization in lymphoid cells: human lymphocytes, lymphoblasts cultivated during 24 h and 44 h, and T cells of Jurkat cell line. Two features of the loop domain organization were found to depend on the cell functional state. First, DNA fraction in the loops of large sizes (more than ~200 kb) was essentially increased in proliferating (de-differentiated) cells in comparison with terminally differentiated lymphocytes. Second, the linear density of the loops not larger than ~200 kb was decreased in transcriptionally active cells and was increased upon their inactivation
Дод.точки доступу:
Afanasieva, K.
Olefirenko, V.
Martyniak, A.
Lukash, L.
Sivolob, A.

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The oxidative status in patients with chronic kidney disease / V. S. Vasylchenko [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P70-77

MeSH-головна:
ПОЧЕК БОЛЕЗНИ -- KIDNEY DISEASES (патофизиология)
ОКСИДАТИВНЫЙ СТРЕСС -- OXIDATIVE STRESS (физиология)
Анотація: An excess of free radicals accompanies the development of renal pathologies and causes numerous concomitant complications and syndromes. The most common of these are cardiometabolic syndromes in patients with chronic kidney disease. Therefore, the purpose of the study was to determine the activity of paraoxonase-1 and myeloperoxidase, which are associated with indicators of high-density lipoproteins content and oxidative stress in the blood of patients with the chronic stage of kidney disease. The activity of the enzymes, thiobarbiturate-active products concentration and transferrin, ceruloplasmin, thiol compounds content were determined in the blood of patients with chronic kidney disease. The oxidative status was shown to be changed. Thus, myeloperoxidase activity, the content of oxidized proteins and the concentration of thiobarbiturate-positive components were increased, while the activity of the antioxidant enzyme paraoxonase-1, the content of transferrin, ceruloplasmin and thiol compounds were decreased. The ratio of myeloperoxidase/paraoxonase-1 activities was progressively increased up to 9-fold, indicating the presence of cardiovascular complications in patients. The data obtained allowed to extend the range of indicators for monitoring the development of cardiometabolic disorders in the progression of chronic kidney disease
Дод.точки доступу:
Vasylchenko, V. S.
Korol, L. V.
Kuchmenko, O. B.
Stepanova, N. M.

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Effects of ethylthiosulfanylate and chromium (VI) on the state of pro/antioxidant system in rat liver / B. I. Kotyk [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P78-86

Рубрики: Этилтиосульфанилат

MeSH-головна:
ПЕЧЕНЬ -- LIVER (патофизиология)
ХРОМ -- CHROMIUM (метаболизм)
Кл.слова (ненормовані):
АНТИОКСИДАНТНАЯ СИСТЕМА
Анотація: Ethylthiosulfanylate is alkyl ester of thiosulfoacid and belongs to the class of thiosulfonate compounds. Structurally, thiosulfonates are synthetic analogues of natural phytoncides. It is known that, natural organic sulfur-containing compounds are characterized by antioxidant and detoxification properties against heavy metals toxicity. Therefore, the purpose of the study was to investigate the influence of ethylthiosulfanylate, as a synthetic analogue of natural phytoncides, on the state of the pro/antioxidant system in the liver of laboratory rats exposed to Cr(VI). It was found that ethylthiosulfanylate exposure at a dose 100 mg/kg body weight daily for 14 days led to a decrease in the intensity of increasing of the lipid hydroperoxides (LHP) content in the rat liver caused by Cr(VI) action. In addition, ethylthiosulfanylate pretreatment prevented depletion of reduced glutathione (GSH) pool under the action of potassium dichromate oxidative stress and performed the accumulation of cellular GSH in rat liver
Дод.точки доступу:
Kotyk, B. I.
Iskra, R. Ya.
Slivinska, O. M.
Liubas, N. M.
Pylypets, A. Z.
Lubenets, V. I.
Pryimych, V. I.

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10.

    Metallothioneins contribution to the response of Bivalve mollusk to xenobiotics / V. V. Khoma [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P87-96

Рубрики: Раундап

   Металлотиониены

MeSH-головна:
МОЛЛЮСКИ ДВУСТВОРЧАТЫЕ -- BIVALVIA (действие лекарственных препаратов)
КСЕНОБИОТИКИ -- XENOBIOTICS (вредные воздействия)
ДИКЛОФЕНАК -- DICLOFENAC (фармакокинетика)
ФЕНИГИДИН -- NIFEDIPINE (вредные воздействия)
Анотація: Estimation of cellular thiols metallothioneins (MTs) sensitivity to continuous pressure of environmental chemical ‘cocktail’ of xenobiotics needs investigation in correct model experiments. The aim of this study was to elucidate MT s contribution into bivalve mollusk response to co-exposure to xenobiotics and elevated temperature. We treated the mussels Unio tumidus Philipson, 1788 (Unionidae) with drugs diclofenac (Dc, 2 nM), nifedipine (Nf, 2 nM) or with organophosphonate herbicide glyphosate (Gl, formulation Roundup MAX, 79 nM) separately at 18°C and in combination at 18°C (DcNfGl) and 25°C (DcNfGl+T) during 14 days. MTs were isolated from digestive gland by size-exclusion chromatography. The concentration of MTs in the tissue was assessed according to metals (Zn, Cu, Cd) in the eluted peak of MTs (MT-Me) and thiols (MT-SH) content. Tissue redox status was assessed using lactate/pyruvate ratio. The assay of cells viability was based on the lysosomes ability of hemocytes to concentrate the Neutral Red (NR) dye. It was found, that MT-SH content in the digestive gland was increased under all exposures. Treatment with Dc increased the level of MT-Me, whereas treatment with Gl decreased it and increased lactate/pyruvate ratio. Nf decreased this ratio by elevating pyruvate level and increased lysosomal membrane stability in hemocytes. At co-exposure to xenobiotics and elevated temperature the number of hemocytes with nuclear abnormalities was increased indicating the exceeding of organisms’ adaptive limits. Multivariate statistical analyses showed negative correlations in pairs MT-SH/MT-Me and MT-SH/pyruvate and distinguished Gl and DcNfGl+T exposed groups from other groups
Дод.точки доступу:
Khoma, V. V.
Gnatyshyna, L. L.
Martyniuk, V. V.
Mackiv, T. R.
Mishchuk, N. Y.
Stoliar, O. B.

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11.

Dietary sucrose defines lifespan and metabolism in Drosophila / O. Strilbytska [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P97-105

MeSH-головна:
ДРОЗОФИЛА -- DROSOPHILA (метаболизм)
ДИЕТА -- DIET (использование)
САХАРОЗА -- SUCROSE
Анотація: Nutrition affects various life-history traits. We used fruit flies Drosophila melanogaster to determine whether life-history traits, particularly life span and metabolism, are affected by dietary sucrose content. We fed flies by four different diets containing constant yeast concentration and increasing amounts of sugar ranged from 1% to 20%. We found that low sucrose diet increases female lifespan. We also showed, that low dietary sucrose maximized malate dehydrogenase, aspartate aminotransferase activity in males and lactate dehydrogenase activity in females. In addition, dietary carbohydrate has a considerable impact on urea level, suggesting that dietary carbohydrate impacts overall metabolism. Our findings reveal the influence of dietary sugar on metabolic enzymes activities, indicating an existence of optimal nutritional conditions for prolongevity phenotype and confirming an important impact of dietary sugar on life-history traits
Дод.точки доступу:
Strilbytska, O.
Strutynska, T.
Semaniuk, U.
Burdyliyk, N.
Lushchak, O.

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12.

Effect of sucralose on the blood content of thyroid hormones / O. Oliynyk [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P106-110

Рубрики: Сукралоза

MeSH-головна:
ЩИТОВИДНАЯ ЖЕЛЕЗА -- THYROID GLAND (действие лекарственных препаратов)
Анотація: Ethylthiosulfanylate is alkyl ester of thiosulfoacid and belongs to the class of thiosulfonate compounds. Structurally, thiosulfonates are synthetic analogues of natural phytoncides. It is known that, natural organic sulfur-containing compounds are characterized by antioxidant and detoxification properties against heavy metals toxicity. Therefore, the purpose of the study was to investigate the influence of ethylthiosulfanylate, as a synthetic analogue of natural phytoncides, on the state of the pro/antioxidant system in the liver of laboratory rats exposed to Cr(VI). It was found that ethylthiosulfanylate exposure at a dose 100 mg/kg body weight daily for 14 days led to a decrease in the intensity of increasing of the lipid hydroperoxides (LHP) content in the rat liver caused by Cr(VI) action. In addition, ethylthiosulfanylate pretreatment prevented depletion of reduced glutathione (GSH) pool under the action of potassium dichromate oxidative stress and performed the accumulation of cellular GSH in rat liver
Дод.точки доступу:
Oliynyk, O.
Slifirczyk, A.
Oliynyk, Y.
Pereviznyk, B.

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13.

Changes in gene expression of lactate carriers (MCT1 and CD147) in cardiac muscle of diabetic male rats: the effect of dichloroacetate and endurance training / H. Rezaeinasab [et al.] // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P111-119

MeSH-головна:
ДИАБЕТ САХАРНЫЙ -- DIABETES MELLITUS (генетика, метаболизм, патофизиология)
МИОКАРД -- MYOCARDIUM (патология)
ДИХЛОРУКСУСНАЯ КИСЛОТА -- DICHLOROACETIC ACID (вредные воздействия)
ВЫНОСЛИВОСТИ ТРЕНИРОВКА -- RESISTANCE TRAINING (вредные воздействия)
Анотація: Lactate accumulation can activate the pathways of mitochondrial biogenesis in the heart muscle. The purpose of this study was to investigate the effects of Pyruvate Dehydrogenase Kinase 4 (PDK4) inhibition and endurance training on the gene expression of lactate carriers (MCT1 and CD147) in the cardiac muscle of STZ-diabetic rats. In this experimental study, 64 male Wistar rats were selected and randomly divided into eight groups after induction of diabetes with streptozotocin (STZ). The endurance training protocol was performed on a treadmill for 6 weeks. Intraperitoneal injection of DCA of 50 mg/ kg body weight was used for the inhibition of PDK4 in the myocardium. Gene expression were measured using real-time PCR. The two-way ANOVA test was used to analyze the data. The results of the study showed that after endurance training, the expression of MCT1, PDK4, and CD147 genes increased significantly in line with each other (P 0.05), and by inhibition of PDK4 in the heart muscle, the expression of MCT1 and CD147 genes in the endurance training group + diabetes + DCA and in the diabetes group + DCA decreased significantly (P 0.05). According to the results of this study, it can be concluded that the repeated accumulation of lactate caused by exercise training in diabetic patients decrease through mitochondrial adaptation by DCA injection and subsequently oxidative stress can be reduced in cardiac tissue of diabetic patients and heart efficacy can be increased
Дод.точки доступу:
Rezaeinasab, H.
Habibi, A.
Nikbakht, M.
Rashno, M.
Shakerian, S.

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14.

Hrushanyk, N. V.
Oxidative stress regulation in the yeast Ogataea polymorpha producer of human α-synuclein / N. V. Hrushanyk, O. V. Stasyk, O. G. Stasyk // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P120-133

MeSH-головна:
ПАРКИНСОНА БОЛЕЗНЬ -- PARKINSON DISEASE (патофизиология)
ДРОЖЖИ -- YEASTS (действие лекарственных препаратов)
ГЛЮКОЗА -- GLUCOSE (метаболизм)
ОКСИДАТИВНЫЙ СТРЕСС -- OXIDATIVE STRESS (физиология)
Анотація: In this study we analyzed how exogenous glucose levels affect enzymatic and non-enzymatic antioxidant defense systems and markers of oxidative stress in cells of the methylotrophic yeast Ogataea polymorpha producing recombinant human α-synuclein, implicated in pathogenesis of neurodegenerative Parkinson’s disease (PD). We found that glucose depletion up-induced activity of antioxidant enzymes superoxide dismutase, and catalase, and increased content of reduced and oxidized glutathione in the cells cultivated in the medium with 0.1% glucose, as compared to physiological growth condition (1% glucose-containing medium). In addition, low glucose concentration in the medium upregulated content of proteins carbonyl groups and of products of lipid peroxidation. Notably, the shift in the equilibrium toward pro-oxidant changes was similar for recombinant α-synuclein producer and parental wild-type strain. Thus, glucose limitation leads to the overproduction of reactive oxygen species in the methylotrophic yeast cells independently of the recombinant human α-synuclein production
Дод.точки доступу:
Stasyk, O. V.
Stasyk, O. G.

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15.

Виноградова, Р. П.
Дослідження структури, механізму та регуляції активності ензимів у роботах Нобелівських лауреатів. К. Анфінсен, С. Мур, В. Стайн, С. Прузинер, Є. Скоу, Д. Бойєр, Д. Вокер / Р. П. Виноградова, B. М. Данилова, С. В. Комісаренко // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - С. 134-154

MeSH-головна:
ФЕРМЕНТЫ -- ENZYMES (метаболизм)
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Анотація: Від часу встановлення протеїнової природи ензимів у 40-і роки ХХ ст., про що йшлося в нашій попередній статті, молекулярна структура і конкретний механізм їх дії залишались невідомими. Ці завдання постали перед дослідниками наступних поколінь, які досягли значних успіхів в їх вирішенні. Так, у 1960 р. американські біохіміки С. Мур і В. Стайн встановили повну послідовність амінокислот в ензимі рибонуклеазі. Це був один із перших протеїнів і перший ензим, в якому було встановлено первинну структуру. За це відкриття в 1972 р. їм було присуджено половину нобелівської премії з хімії; другу половину за вирішення цієї ж проблеми отримав Крістіан анфінсен. Роботи нобелівських лауреатів з хімії за 1997 р. – Єнса Крістіана Скоу (відкриття ензиму Na+,K+-активованої аТРази) та Пола Бойєра і джона Вокера (відкриття механізму дії н+-аТ Р-синтази – найважливішого ензиму біо-енергетики) були величезним кроком вперед у розшифруванні механізму дії ензимів – найважливіших компонентів метаболізму в живих організмах. друга половина ХХ ст. відзначена ще одним видатним відкриттям у галузі біології та медицини – виявленням і дослідженням протеїнів – пріонів, які спри-чинюють спонгіоформні нейродегенеративні енцефалопатії в людей та тварин, за яке американський біохімік Стенлі В. Прузинер у 1997 р. отримав нобелівську премію з фізіології і медицини. Це від-криття має велике теоретичне значення для біохімічної науки. Розробка нових методів дослідження і особливо їх апаратурне оформлення стали основою для розвитку цих робіт із хімії протеїнів, що привело до значних наукових досягнень в цій галузі біохімії та молекулярної біології – «золотої ери» біохімії протеїнів
Although the protein nature of enzymes was identified in the 40s of the 20th century, (we wrote about this in our previous article), their molecular structure and the specific mechanism of action remained unknown. Researchers of the next generations faced the challenges and a major breakthrough was achieved. In 1960, American biochemists S. Moore and W. Stein determined the complete amino acid sequence of enzyme ribonuclease. It was one of the first proteins and the first enzyme whose primary structure was established. In 1972, for this discovery, they received the Nobel Prize in Chemistry jointly to Christian Anfinsen who worked on the same problem. Works of Nobel Laureates in Chemistry in 1997 – Jens Christian Skou (for the discovery of the Na+,K+-activated ATPase), Paul Boyer and John Walker (for the discovery of the mechanism of action of H+-ATP synthase – the most important enzyme for bioenergy) were a huge step forward in the deciphering the mechanisms of enzyme action. The second half of the 20th century was marked by another outstanding discovery in the field of biology and medicine – the identification and characterization of prions – the proteins that cause neurodegenerative spongiform encephalopathies in humans and animals. For this work, American biochemist Stanley B. Prusiner received the Nobel Prize in Physiology or Medicine in 1997. This discovery is of great theoretical significance for biochemical science. The development of new research methods and technological advances formed the basis for significant scientific achievements in this field of biochemistry and molecular biology. This was the golden era of protein chemistry
Дод.точки доступу:
Данилова, В. М.
Комісаренко, С. В.
Анфінсен, Крістіан Бемер (26.03.1916-14.05.1995)
Мур, Станфорд (4.09.1913-23.08.1982)
Стайн, Вільям Говард (25.06.1911-2.02.1980)
Прузинер, Стенлі (28.05.1942)
Скоу, Єнс Крістіан (8.10.1918-28.05.2018)
Бойєр, Пол Делос (31.07.1918-2.06.2018)
Вокер, Джон Ернест (7.01.1941)

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16.

Григор’єва, М. В.
Новий погляд на РНК: відкриття Сідні Олтмена і Томаса Чека 1989 року / М. В. Григор’єва, B. М. Данилова, С. В. Комісаренко // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - С. 155-160

MeSH-головна:
РНК -- RNA (анализ, генетика, классификация, стандарты)
Анотація: Нобелівським лауреатам із хімії 1989 р. Сідні олтмену та Томасу Чеку належить одне з найважливіших відкриттів у молекулярній генетиці. незалежно один від одного вони продемонстрували нові експериментальні докази того, що молекули рнК можуть не просто передавати генетичну інформацію від ДнК, але й за певних умов проявляти ензиматичні (каталітичні) властивості. Виявилося, що функції, виконання яких раніше відводили виключно протеїновим ензимам, можуть виконувати також і рнК, названі рибозимами. Згодом рибозими стали новим інструментом в генній інженерії, біохімії, біотехнології та медицині
The 1989 Nobel Prize Laureates in Chemistry Sidney Altman and Thomas Robert Cech made one of the most important discoveries in molecular genetics. Independently of each other, they demonstrated new experimental evidence that RNA molecules can not only transmit information from DNA but, in certain conditions, have the enzymatic (catalytic) properties too. As it turned out, the functions previously attributed exclusively to protein enzymes can be also performed by RNA called ribozymes. Later, ribozymes became a new tool in genetic engineering, biochemistry, biotechnology and medicine
Дод.точки доступу:
Данилова, В. М.
Комісаренко, С. В.
Олтмен, Сідні (1939)
Чек, Томас (8.12.1947)

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17.

Ebrahimi, M.
A glance on the role of Hsien Wu in immunology development / M. Ebrahimi // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P161-165

MeSH-головна:
АЛЛЕРГОЛОГИЯ И ИММУНОЛОГИЯ -- ALLERGY AND IMMUNOLOGY (история, кадры)
Анотація: Early in the 20th century, a number of researchers in the field of immunology investigated this science chemically. Immunochemistry is the study of antigens and antibodies and their chemical basis and resistance to disease, developed from immunology. The immunochemistry period began in 1918 and continued through the early 1960s. Since the beginning of the immunochemical period, many researchers have been working in the field of immunochemistry by introducing important immunohistochemical and immunocytochemical methods. Hsien Wu was inspired by the science of immunochemistry and was able to determine a method for the determination of hemoglobin. In this article, I attempt to illustrate Hsien’s achievements in this period by presenting Hsien Wu’s scientific biography and immunochemical history. Furthermore, providing documentary and scientific information on the course of immunochemistry and the role of Hsien in this course may be a spark for some researchers to explore the reasons for some of the chemical approaches and theories of this period
Дод.точки доступу:
Hsien, Wu
Сянь, Ву

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18.

The Nobel Prize in Physiology or Medicine 2020 // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P166-167

MeSH-головна:
ФИЗИОЛОГИЯ -- PHYSIOLOGY (история, кадры)
МЕДИЦИНА -- MEDICINE
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
Дод.точки доступу:
Alter, Harvey James (12.09.1935) \о нем\
Houghton, Michael (1949) \о нем\
Rice, Ch. M. 25.09.1952 \о нем\

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19.

The Nobel Prize in Chemistry 2020 // The Ukrainian biochemical journal. - 2020. - Vol. 92, № 5. - P168

MeSH-головна:
НОБЕЛЕВСКАЯ ПРЕМИЯ -- NOBEL PRIZE
ХИМИЯ -- CHEMISTRY (история, кадры)
Дод.точки доступу:
Charpentier, Emmanuelle Marie (11.12.1968) \о ней\
Doudna, Jennifer Anne (19.02.1964) \о ней\

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© Міжнародна Асоціація користувачів і розробників електронних бібліотек і нових інформаційних технологій
(Асоціація ЕБНІТ)